What is the purpose of primers in the PCR technique?

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Primers play a crucial role in the PCR (Polymerase Chain Reaction) technique by binding to specific regions of the template DNA. They are short sequences of nucleotides that are designed to complement the sequences at the start of the target region of the DNA that you want to amplify. When the PCR process begins, the temperature is raised to denature the DNA, resulting in two single strands. As the temperature is lowered, the primers anneal, or bind, to their complementary sequences on the single-stranded DNA. This binding provides a starting point for DNA polymerase to extend the new DNA strand during the elongation phase of PCR. Because the primers dictate the specificity of the PCR, they are essential for ensuring that only the desired segment of DNA is amplified.

In other contexts, while stabilization and other functions are important in molecular biology, the unique purpose of primers in PCR is specifically to provide binding sites for the DNA polymerase, which is not addressed adequately by the other options.

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